基于光热-激光背向散射干涉的生物分子定量检测技术Quantitative detection technique of biomolecule based on photothermal effect and laser backscattering interferometry
李荣;张校亮;谭慷;李晓春;
摘要(Abstract):
针对传统的显色法中显色产物的定量检测问题,发展了1种结合光热效应和激光背向散射干涉(back-scattering interferometry,BSI)的生物分子定量检测技术。发生显色反应后的生物分子、离子溶液吸收激光会产生光热效应,使得溶液折射率发生改变,其改变量与待测的分子、离子浓度在一定范围内呈正相关。利用激光背向散射干涉技术可实现折射率变化的灵敏检测,从而实现待测物质浓度的定量检测。文中以亚硝酸盐为例,利用改进的格里斯重氮化反应,使得亚硝酸盐中的NO2-转变为紫红色偶氮化合物。采用波长为532nm的激光,利用显色反应后溶液的光热效应和BSI技术实现了NO2-质量浓度的定量检测,使用3σ方法得到检测限为0.05mg/L。
关键词(KeyWords): 分析化学;光热效应;背向散射干涉;亚硝酸盐;定量检测
基金项目(Foundation): 国家自然科学基金资助项目(11504259,21505098,21575098);; 山西省国际合作资助项目(2015081019);; 山西省高等学校科技创新资助项目(2015123);; 山西省平台基地和人才专项资助项目(201605D211033)
作者(Author): 李荣;张校亮;谭慷;李晓春;
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DOI:
参考文献(References):
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